期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 323, 期 2, 页码 101-108出版社
ELSEVIER
DOI: 10.1016/j.jim.2007.03.005
关键词
dendritic cell; serum-free; plasmacytoid DC; myeloid DC; tolerance
资金
- NIAID NIH HHS [P01 AI051392-06, P01 AI051392] Funding Source: Medline
Standard protocols for the generation of murine dendritic cells (DCs) employ medium supplemented with heat-inactivated fetal calf serum (FCS). Recently, several attempts have been made to avoid serum exposure during DC culture. The impetus for these efforts has been a desire to generate DCs for clinical use, as preclinical data have demonstrated their efficacy in immune activation and in immune suppression both in vitro and in vivo. However, these protocols have resulted in contradictory outcomes with respect to DC survival in culture and activation status. In this report, we compared several serum-free culture conditions with respect to survival, differentiation, activation, and cytokine profile of murine DC progenitors. DC progenitors can survive only in some serum-free conditions. Surprisingly, DCs grown in serum-free medium display a higher expression of activation markers upon stimulation. They produce increased IL-12 and decreased IL-6 following stimulation. Furthermore, DCs derived under serum-free conditions may express unusual surface markers, B220 and Ly6C/G, implying an increased differentiation to plasmacytoid DCs (pDCs). (C) 2007 Elsevier B.V. All rights reserved.
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