期刊
ANALYTICAL CHEMISTRY
卷 85, 期 3, 页码 1642-1649出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac302910x
关键词
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资金
- Ministry of Science and Technology of China [MOST 2011IM030100]
- University of Sheffield
Accurately measuring carbon flows is a challenge for understanding processes such as diverse intracellular metabolic pathways and predator-prey interactions. Combined with stable isotope probing (SIP), single-cell Raman spectroscopy was demonstrated for the first time to link the food chain from carbon substrate to bacterial prey up to predators at the single-cell level in a quantitative and nondestructive manner. Escherichia coli OP50 with different C-13 content, which were grown in a mixture of C-12- and fully carbon-labeled C-13-glucose (99%) as a sole carbon source, were fed to the nematode. The C-13 signal in Caenorhabditis elegans was proportional to the C-13 content in E. coli. Two Raman spectral biomarkers (Raman bands for phenylalanine at 1001 cm(-1) and thymine at 747 cm(-1) Raman bands), were used to quantify the C-13 content in E. coli and C. elegans over a range of 1.1-99%. The phenylalanine Raman band was a suitable biomarker for prokaryotic cells and thymine Raman band for eukaryotic cells. A biochemical mechanism accounting for the Raman red shifts of phenylalanine and thymine in response to C-13-labeling is proposed in this study and is supported by quantum chemical calculation. This study offers new insights of carbon flow via the food chain and provides a research tool for microbial ecology and investigation of biochemical pathways.
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