期刊
ANALYTICAL CHEMISTRY
卷 85, 期 21, 页码 10107-10116出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac400996x
关键词
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资金
- Swiss National Foundation [200021_119779, 31003A_143877]
- Swiss National Science Foundation (SNF) [PP00P3_144874]
- Swiss National Science Foundation (SNF) [31003A_143877, 200021-119779, PP00P3_144874] Funding Source: Swiss National Science Foundation (SNF)
In recent years, chemical imaging was prognosticated to become one of the key analytical applications for laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). However, moderate spatial resolution and the associated measurement time required for a larger sampling area, have restricted this versatile, high sensitivity technique from being routinely used in two-dimensional chemical imaging. This work describes the development and investigation of a low dispersion sample chamber (tube cell), which allows improvement of the imaging capabilities by reduction of the single LA shot duration to 30 ms (full width at 1% maximum). The new tube cell is based on a constant laminar flow and a well-controlled delivery of the laser-ablated aerosol into the transport system, leading to minimized tailing of the aerosol washout and helping to separate the signals even at repetition rates as high as 20-30 Hz. To demonstrate the improved imaging capabilities, microstructured metallic thin film patterns were analyzed at a spatial resolution of a few micrometers. The LA-ICP-MS results obtained were comparable to Synchrotron-based micro-X-ray fluorescence (SR-microXRF). The suitability of the newly designed cell for multielement acquisitions was demonstrated using a simultaneous ICP-Mattauch-Herzog-MS. Finally, the novel laser ablation cell was applied to image the distribution of a metal-tagged biomarker in a thin section of breast cancer tissue. This application demonstrates that the technique is able to produce subcellular (similar to 1 mu m) spatial resolution, which is crucial for morphological assessment in cancer diagnostics.
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