期刊
ANALYTICAL CHEMISTRY
卷 85, 期 20, 页码 9713-9720出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac402118a
关键词
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资金
- Department of the Defense, Defense Threat Reduction Agency [HDTRA-1-13-1-0031]
- Robert A. Welch Foundation [F-0032, F-1654]
- NIH [U54EB015403-01]
We demonstrate the hybridization-induced fluorescence detection of DNA on an origami-based paper analytical device (oPAD). The paper substrate was patterned by wax printing and controlled heating to construct hydrophilic channels and hydrophobic barriers in a three-dimensional fashion. A competitive assay was developed where the analyte, a single-stranded DNA (ssDNA), and a quencher-labeled ssDNA competed for hybridization with a fluorophore-labeled ssDNA probe. Upon hybridization of the analyte with the fluorophore-labeled ssDNA, a linear response of fluorescence vs analyte concentration was observed with an extrapolated limit of detection <5 nM and a sensitivity relative standard deviation as low as 3%. The oPAD setup was also tested against OR/AND logic gates, proving to be successful in both detection systems.
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