期刊
ANALYTICAL CHEMISTRY
卷 85, 期 13, 页码 6505-6510出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac401105p
关键词
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资金
- National Basic Research Program of China [2010CB732400]
- National Natural Science Foundation of China [21075060, 21135002, 21121091]
- Excellent Talents in Chinese Universities [NCET100479]
A modular labeling strategy was presented for electrochemical immunoassay via supramolecular host-guest interaction between beta-cyclodextrin (beta-CD) and adamantine (ADA). An ADA-labeled antibody (ADA-Ab) was synthesized via amidation, and the number of ADA moieties loaded on a single antibody was calculated to be similar to 7. The beta-CD-functionalized gold-palladium bimetallic nanoparticles (AuPd-CD) were synthesized in aqueous solution via metal-S chemistry and characterized with transmission electron microscopy and X-ray photoelectron spectra. After the ADA-Ab was bound to the antigen-modified electrode surface with a competitive immunoreaction, AuPd-CD as a signal tag was immobilized onto the immunosensor by a host-guest interaction, leading to a large loading of AuPd nanoparticles. The highly efficient electrocatalysis by AuPd nanoparticles for NaBH4 oxidation produced an ultrasensitive response to chloramphenicol as a model of a small molecule antigen. The immunoassay method showed a wide linear range from 50 pg/mL to 50 mu g/mL and a detection limit of 4.6 pg/mL. The specific recognition of antigen by antibody resulted in good selectivity for the proposed method. The host-guest interaction strategy provided a universal labeling approach for the ultrasensitive detection of small molecule targets.
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