期刊
ANALYTICAL CHEMISTRY
卷 85, 期 13, 页码 6279-6286出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac4004437
关键词
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资金
- National Basic Research Program of China (973 program) [2009CB421601, 2011CB911002]
- Foundation for Innovative Research Groups of NSFC [21221003]
- National Natural Science Foundation of China [21222507, 21175036, 21190044, 21075031]
- Program for New Century Excellent Talents in University [NCET-10-0366]
- Natural Science Foundation of Hunan Province [10JJ2005]
In this paper, the efficient quenching effect of deoxyguanosine-S'-phosphate (dGMP) on anodic electrochemiluminescence (ECL) of the CdTe/ZnS quantum dots (QDs) is reported for the first time. This ECL quenching was found to be specific for free dGMP and not observed for dGMP residues in different DNA structures. The unique dGMP-based QDs ECL quenching was then utilized to develop a versatile biosensing strategy to determine various protein-DNA interactions with the assistance of exonuclease, Exo I, to hydrolyze DNA and liberate dGMP. Taking single-stranded DNA binding protein (SSB) and thrombin as examples, two novel detection modes have been developed based on dGMP-QDs ECL strategy. The first method used hairpin probes and SSB-promoted probe cleavage by Exo I for facile signal-off detection of SSB, with a wide linear range of 1-200 nM and a low detection limit of 0.1 nM. The second method exploited aptamer-thrombin binding to protect probes against Exo I degradation for sensitive signal-on detection of thrombin, giving a linear response over a range of 1-150 nM and a detection limit as low as 0.1 nM. Both methods were homogeneous and label free without QDs or DNA modification. Therefore, this dGMP-specific QDs ECL quenching presents a promising detection mechanism suitable for probing various protein-nucleic acid interactions.
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