期刊
ANALYTICAL CHEMISTRY
卷 85, 期 18, 页码 8483-8487出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac401668e
关键词
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资金
- National Basic Research Priorities Program [2012CB910602, 2013CB911201]
- National Natural Science Foundation of China [21075022, 20875017, 21105016]
- Research Fund for the Doctoral Program of Higher Education of China [20110071110007, 20100071120053]
- Shanghai Municipal Natural Science Foundation [11ZR1403200]
- Shanghai Leading Academic Discipline Project [B109]
To discover trace phosphorylated proteins or peptides with great biological significance for in-depth phosphoproteome analysis, it is urgent to develop a novel technique for highly selective and effective enrichment of phosphopeptides. In this work, an IMAC (immobilized metal ion affinity chromatography) material with polydopamine coated on the surface of graphene and functionalized with titanium ions (denoted as Ti4+-G@PD) was initially designed and synthesized The newly prepared Ti4+-G@PD with enhanced hydrophilicity and biological compatibility was characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and infrared (IR), and its performance for selective and effective enrichment of phosphopeptide was evaluated with both standard peptide mixtures and human serum.
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