期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 16, 期 10, 页码 25678-25690出版社
MDPI
DOI: 10.3390/ijms161025678
关键词
bone tissue engineering; alpha-TCP; CS; inflammatory response; RANKL; TNF-alpha; osteoclastogenesis; interferon-gamma; zoledronate
资金
- Osaka Dental University Research Funds in Japan [15-08]
- Grants-in-Aid for Scientific Research [25463041, 25463062, 25463061] Funding Source: KAKEN
Inflammatory responses are frequently associated with the expression of inflammatory cytokines and severe osteoclastogenesis, which significantly affect the efficacy of biomaterials. Recent findings have suggested that interferon (IFN)- and zoledronate (Zol) are effective inhibitors of osteoclastogenesis. However, little is known regarding the utility of IFN- and Zol in bone tissue engineering. In this study, we generated rat models by generating critically sized defects in calvarias implanted with an alpha-tricalcium phosphate/collagen sponge (-TCP/CS). At four weeks post-implantation, the rats were divided into IFN-, Zol, and control (no treatment) groups. Compared with the control group, the IFN- and Zol groups showed remarkable attenuation of severe osteoclastogenesis, leading to a significant enhancement in bone mass. Histomorphometric data and mRNA expression patterns in IFN- and Zol-injected rats reflected high bone-turnover with increased bone formation, a reduction in osteoclast numbers, and tumor necrosis factor- expression. Our results demonstrated that the administration of IFN- and Zol enhanced bone regeneration of -TCP/CS implants by enhancing bone formation, while hampering excess bone resorption.
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