期刊
ANALYTICAL CHEMISTRY
卷 85, 期 17, 页码 8055-8060出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac4018988
关键词
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资金
- NSF
- Burroughs Wellcome Fund Career Award
- James H. Ferry Fund for Innovation
- Joseph R. Mares endowed chair
Many studies have demonstrated the concept of using free-radical polymerization reactions to provide signal amplification so that molecular recognition events indicative of disease states may be detected in a simple and low-cost manner. We provide the first systematic study of how the dissociation constant impacts detection sensitivity in these assays, having chosen a range of dissociation constants (nanomolar to picomolar) that is typical of those encountered in molecular diagnostic applications that detect protein protein binding events. In addition, we use experimental results to validate a mass-action kinetic model that may be used to predict assay performance as an alternative or supplement to the empirical approach to developing new polymerization-based amplification assays that has characterized the field to date.
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