期刊
ANALYTICAL CHEMISTRY
卷 85, 期 5, 页码 2645-2651出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac302903p
关键词
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资金
- National Basic Research Program of China (973 Program) [2011CB933600]
- Science Fund for Creative Research Groups of NSFC [20921062]
- National Natural Science Foundation of China [20875072, 21175100]
- Program for New Century Excellent Talents in University [NCET-10-0656]
- 3551 Talent Program of the Administrative Committee of East Lake Hi-Tech Development Zone [[2011]137]
In this work, we reported a simple rapid and point-of-care magnetic immunofluorescence assay for avian influenza virus (AIV) and developed a portable experimental setup equipped with an optical fiber spectrometer and a microfluidic device. We achieved the integration of immunomagnetic target capture, concentration, and fluorescence detection in the microfluidic chip. By optimizing flow rate and incubation time, we could get a limit of detection low up to 3.7 x 10(4) copy/mu l, with a sample consumption of 2 mu L and a total assay time of less than SS min. This approach had proved to possess high portability, fast analysis, high specificity, high precision, and reproducibility with an intra-assay variability of 2.87% and an interassay variability of 4.36%. As a whole, this microfluidic system may provide a powerful platform for the rapid detection of AIV and may be extended for detection of other viral pathogens; in addition, this portable experimental setup enables the development of point-of-care diagnostic systems while retaining adequate sensitivity.
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