期刊
ANALYTICAL CHEMISTRY
卷 85, 期 24, 页码 11686-11690出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac403691a
关键词
-
资金
- 973 Program [2012CB932600]
- National Natural Science Foundation of China [21025522, 21135003, 21121091, 21305063]
- Natural Science Funds of Jiangsu Province [BK20130553]
To date, almost all the established photo-electrochemical (PEC) enzymatic biosensors require the surface-confinement procedure to immobilize enzyme as biorecogniton element for probing various analytes of interest. This Letter develops a novel example without such necessity. Specifically, we first prepared a BiOI nanoflakes (NFs)/TiO2 nanoparticles (NPs) p-n heterojunction as the photo-electrode, on the basis of which acetylcholine esterase (AChE) antibody was introduced via the bridging of protein A. In such a system, enzyme could keep its optimal state in the solution if in the absence of inhibitor; otherwise, the degree of enzyme inhibition would correlate closely with the concentration of inhibitor. After immunoreaction between AChE and its antibody, the inhibitor concentration could then be determined by the biocatalytic reaction-controlled PEC response. Integrated with other enzyme-based biosystems, this simple configuration could serve as a general method for assaying enzyme inhibition or activities.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据