期刊
ANALYTICAL CHEMISTRY
卷 85, 期 21, 页码 10432-10439出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac402415v
关键词
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资金
- NSF [CHE-1150969]
- Eli Lilly
- Alfred P. Sloan Research Fellows Program
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1150969] Funding Source: National Science Foundation
This Article describes a strategy for quantifying active enzyme analytes in a paper-based device by measuring the time for a reference region in the paper to turn green relative to an assay region. The assay requires a single step by the user, yet accounts for variations in sample volume, assay temperature, humidity, and contaminants in a sample that would otherwise prevent a quantitative measurement. The assay is capable of measuring enzymes in the low to mid femtomolar range with measurement times that range from similar to 30 s to similar to 15 min (lower measurement times correspond to lower quantities of the analyte). Different targets can be selected in the assay by changing a small molecule reagent within the paper-based device, and the sensitivity and dynamic range of the assays can be tuned easily by changing the composition and quantity of a signal amplification reagent or by modifying the configuration of the paper-based microfluidic device. By tuning these parameters, limits-of-detection for assays can be adjusted over an analyte concentration range of low femtomolar to low nanomolar, with dynamic ranges for the assays of at least 1 order of magnitude. Furthermore, the assay strategy is compatible with complex fluids such as serum.
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