期刊
GLYCOBIOLOGY
卷 17, 期 7, 页码 677-687出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwm033
关键词
oligosaccharides; MALDI-TOF MS; isotopic labeling; permethylation
资金
- NCRR NIH HHS [P41RR 018502] Funding Source: Medline
Analysis of oligosaccharides by mass spectrometry (MS) has enabled the investigation of the glycan repertoire of organisms with high resolution and sensitivity. It is difficult, however, to correlate the expression of glycosyltransferases with the glycan structures present in a particular cell type or tissue because the use of MS for quantitative purposes has significant limitations. For this reason, in order to develop a technique that would allow relative glycan quanti. cation by MS analysis between two samples, a procedure was developed for the isotopic labeling of oligosaccharides with C-13-labeled methyl iodide using standard permethylation conditions. Separate aliquots of oligosaccharides from human milk were labeled with C-12 or C-13 methyl iodide; the labeled and non-labeled glycans were mixed in known proportions, and the mixtures analyzed by MS. Results indicated that the isotopic labeling described here was capable of providing relative quantitative data with a dynamic range of at least two orders of magnitude, adequate linearity, and reproducibility with a coefficient of variation that was 13% on average. This procedure was used to analyze N-linked glycans released from various mixtures of glycoproteins, such as a-1 acid glycoprotein, human transferrin, and bovine fetuin, using MS techniques that included matrix assisted laser desorption ionization-time of. ight MS and electrospray ionization with ion cyclotron resonance- Fourier transformation MS. The measured C-12: C-13 ratios from mixtures of glycans permethylated with either (CH3I)-C-12 or (CH3I)-C-13 were consistent with the theoretical proportions. This technique is an effective procedure for relative quantitative glycan analysis by MS.
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