期刊
ANALYTICAL CHEMISTRY
卷 85, 期 9, 页码 4357-4362出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac3034568
关键词
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资金
- National Science Foundation (NSF) [0645960, OCE-01751733]
- NSF Science and Technology Center Award [IF0424599]
- Gordon and Betty Moore Foundation
- Direct For Education and Human Resources
- Division Of Graduate Education [0645960] Funding Source: National Science Foundation
Organic ligands dominate the speciation of iron in the ocean. Little is known, however, about the chemical composition and distribution of these compounds. Here, we describe a method to detect low concentrations of organic Fe ligands using reverse-phase high-performance liquid chromatography (HPLC) tandem multicollector inductively coupled plasma mass spectrometry. This technique can be used to screen seawater and marine cultures for target compounds that can be isolated and structurally characterized. Sensitive detection (<1 picomole Fe) is achieved using an iron-free HPLC system to reduce background Fe levels, minimizing (ArO+)-Ar-40-O-16) interferences on Fe-56 with a hexapole collision cell, and introducing oxygen into the sample carrier gas to prevent the formation of reduced carbon deposits that decrease sensitivity. This method was tested with a chromatographic separation of five trace metal complexes that represent the polarity range likely found in seawater. Good separation was achieved with a 20 min water/methanol gradient, although sensitivity decreased by a factor of 2 at high organic solvent concentrations Finally, Fe ligand complexes were detected from the organic extract of surface South Pacific seawater and from culture media of the siderophore producing cyanobacteria Synechococcus sp. PCC 7002.
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