期刊
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
卷 36, 期 6, 页码 669-674出版社
SPRINGER
DOI: 10.1007/s00249-007-0133-7
关键词
single-molecule fluorescence spectroscopy; alternating-laser excitation (ALEX); Forster resonance energy transfer (FRET); biomolecular interactions
类别
资金
- EPSRC [EP/D058775/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/D058775/1] Funding Source: researchfish
- NIGMS NIH HHS [R01 GM069709, GM069709-01, R01 GM069709-01A1] Funding Source: Medline
Alternating-laser excitation (ALEX) spectroscopy has recently been added to the single-molecule spectroscopy toolkit. ALEX monitors interaction and stoichiometry of biomolecules, reports on biomolecular structure by measuring accurate Forster resonance energy transfer (FRET) efficiencies, and allows sorting of subpopulations on the basis of stoichiometry and FRET. Here, we demonstrate that a simple combination of one continuous-wave donor-excitation laser and one directly modulated acceptor-excitation laser (Periodic Acceptor eXcitation) is sufficient to recapitulate the capabilities of ALEX while minimizing the cost and complexity associated with use of modulation techniques.
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