期刊
ANALYTICAL CHEMISTRY
卷 84, 期 19, 页码 8364-8370出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac301918t
关键词
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资金
- National Basic Research Program of China (973 Program) [2011CB933600]
- National Natural Science Foundation of China [20975013]
- Program for New Century Excellent Talents in University [NCET-08-0046]
Real-time tracking of the dynamic process of virus invasion is crucial to understanding the infection mechanism. For successful tracking, efficient labeling methods are indispensable. In this paper, we report a mild and reliable method for labeling viruses, especially with regard to easily disabled enveloped viruses. The copper-free click chemistry has been used to label enveloped viruses with quantum dots (QDs) by linking virions modified with azide to the QDs derived with dibenzocyclooctynes (DBCO). Both vaccinia virus (VACV) and avian influenza A virus (H9N2) can be specifically and rapidly labeled under mild conditions, with a labeling efficiency of more than 80%. The labeled virions were of intact infectivity, and their fluorescence was strong enough to realize single-virion tracking. Compared to previously reported methods, our method is less destructive, reliable, and universal, without specific requirements for the type and structure of viruses to be labeled, which has laid the foundation for long-term dynamic visualization of virus infection process.
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