期刊
ANALYTICAL CHEMISTRY
卷 84, 期 4, 页码 2062-2066出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac202648w
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资金
- Prostate Cancer Foundation
- NU Cancer Center for Nanotechnology Excellence (CCNE) from the National Cancer Institute [U54CA151880]
We report the development of the multiplexed nanoflare, a nanoparticle agent that is capable of simultaneously detecting two distinct mRNA targets inside a living cell. These probes are spherical nucleic acid (SNA) gold nanoparticle (Au NP) conjugates consisting of densely packed and highly oriented oligonucleoticle sequences, many of which are hybridized to a reporter with a distinct fluorophore label and each complementary to its corresponding mRNA target. When multiplexed nanoflares are exposed to their targets, they provide a sequence specific signal in both extra- and intracellular environments. Importantly, one of the targets can be used as an internal control, improving detection by accounting for cell-to-cell variations in nanoparticle uptake and background. Compared to single-component nanoflares, these structures allow one to determine more precisely relative mRNA levels in individual cells, improving cell sorting and quantification.
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