Multiplexed Fluorescence Resonance Energy Transfer Aptasensor between Upconversion Nanoparticles and Graphene Oxide for the Simultaneous Determination of Mycotoxins

We presented a new aptasensor for mycotoxins, which was based on multiplexed fluorescence resonance energy transfer (FRET) between. multicolor upconversion fluorescent nanopartides (UCNPs) as donors and graphene oxide (GO) as the entire and effective acceptor. BaY0.78F5:Yb-0.2, Er-0.02 and BaY0.78F5:Yb-0.7, Tm-0.02 upconversion nanopartides were synthesized and functionalized, respectively, with immobilized ochratoxin A (OTA)-aptamers and fumonisin B-1 (FB1)-aptamers. On the basis of the strong pi-pi stacking effect between the nucleobases of the aptamers and the sp(2) atoms of GO, the aptamer modified-UCNPs can be brought in close proximity to the GO surface. The strong upconversion fluorescence both of BaY0.78F5:Yb-0.2, ET0.02 and BaY0.78F5:Yb-0.2 Tm-0.02 can be completely quenched by the GO, because of a good overlap between the fluorescence emission of multicolor UCNPs and the absorption spectrum of. GO. In contrast, in the presence of OTA and FBI, the aptamers preferred to. bind to their corresponding mycotoxins, which led to changes in the formation of aptamers, and therefore, aptamer modified-UCNPs were far away from the GO surface. Our study results showed that the fluorescence intensity of BaYF5:Yb Er and BaYF5:Yb Tin were related to the concentration Of OTA and FBI. We therefore developed a sensitive and simple platform for the. simultaneous detection of OTA and FB1 with multicolor UCNPs and GO as the FRET pair. The aptasensor provided a linear. range from 0.05 to 100 ng.mL(-1) for OTA and 0.1 to 500 ng.mL(-1) for FB1; the detection limit of OTA was 0.02 ng.mL(-1) and FB1 was 0.1 ng.mL(-1). As a practical application, the aptasensor was used to monitor OTA and FB1 level in naturally contaminated maize samples with the results consistent with that of a classic ELISA method. More importantly, the novel multiplexed FRET. was established for the first time based on multiplexed energy donors to the entire energy acceptor,. this work was expected to open up a new field of FRET system applications for various targets.