期刊
ANALYTICAL CHEMISTRY
卷 84, 期 18, 页码 7670-7676出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac3006878
关键词
-
资金
- United States Army, Medical Research and Materiel Command [W81XWH-05-2-0064, W81XWH-10-1-0358]
- Tennessee Technology Development Corporation
- UTHSC from Research to Prevent Blindness, New York, NY
- Plough Foundation, Memphis, TN
Propofol is a widely used, potent intravenous anesthetic for ambulatory anesthesia and long-term sedation. The target steady state concentration of propofol in blood is 0.25-10 mu g/mL (1-60 mu M). Although propofol can be oxidized electrochemically, monitoring its concentration in biological matrixes is very challenging due to (i) low therapeutic concentration, (ii) high concentrations of easily oxidizable interfering compounds in the sample, and (iii) fouling of the working electrode. In this work we report the performance characteristics of an organic film coated glassy carbon (GC) electrode for continuous monitoring of propofol. The organic film (a plasticized PVC membrane) improved the detection limit and the selectivity of the voltammetric sensor due to the large difference in hydrophobicity between the analyte (propofol) and interfering compounds of the sample, e.g., ascorbic acid (AA) or p-acetamidophenol (APAP). Furthermore, the membrane coating prevented electrode fouling and served as a protective barrier against electrode passivation by proteins. Studies revealed that sensitivity and selectivity of the voltammetric method is greatly influenced by the composition of the PVC membrane. The detection limit of the membrane-coated sensor for propofol in PBS is reported as 0.03 +/- 0.01 mu M. In serum-like electrolyte solutions containing physiologically relevant levels of albumin (5%) and 3 mM AA and 1 mM APAP as interfering agents, the detection limit was 0.5 +/- 0.4 mu M. Both values are below the target concentrations used clinically during anesthesia or sedation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据