期刊
ANALYTICAL CHEMISTRY
卷 84, 期 7, 页码 3170-3178出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac203112c
关键词
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资金
- Deutsche Forschungsgemeinschaft (DFG) [BE 2649/5-1]
- National Institutes of Health [RC2DA028981, 1R01GM092218-01]
- US Defense Threat Reduction Agency [HDTRA1-09-1-0013]
- Vanderbilt University College of Arts and Sciences
- Vanderbilt Institute of Chemical Biology
- Vanderbilt Institute for Integrative Biosystems Research and Education
- VU College of Arts and Sciences
- Sciences Disseration Enhancement Grant
Several complementary mass spectrometric imaging techniques allow mapping of various analytes within biological tissue sections. Laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) quantitatively detects elements and isotopes with very high sensitivity and a particularly high dynamical range. Matrix-assisted laser desorption/ionization ion mobility mass spectrometry (MALDI-IM-MS) allows a pixel-by-pixel classification and identification of bio-molecules. In order to dispose of the healthy hemisphere as an internal calibrant in addition to routinely used external standards, adjacent brain sections of mice with a unilateral 6-OHDA lesion of the medial forebrain bundle were chosen as exemplary samples. We demonstrate a comprehensive way of data acquisition and analysis by coregistering mass spectrometric data on photomicrographs as common reference space and thus providing trimodal spatial information. Registering subsequent planar element maps yielded continuous 3-dimensional data sets. Furthermore, we introduce a correction of MSI data for variable slice thickness applicable to all MSI techniques. In the present case, we observed increased concentrations of iron, manganese, and copper in the lesioned substantia nigra while monounsaturated lipid levels were decreased in the identical region of interest. Our techniques provide new insights into the intricate spatial relationship of morphology and chemistry within tissue.
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