期刊
ANALYTICAL CHEMISTRY
卷 84, 期 16, 页码 6948-6955出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac300372p
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资金
- European Communitys Seventh Framework Programme [223328]
- Deutsche Forschungsgemeinschaft (DFG)
- Bundesministerium fir Bildung und Forschung (BMBF)
- Frankfurt Initiative for Microbial Sciences (FIMS)
Although sharing a certain degree of structural uniformity, natural product classes exhibit variable functionalitics such as different amino acid or acyl residues. During collision induced dissociation, some natural products exhibit a conserved fragmentation pattern close to the precursor ion. The observed fragments result from a shared set of neutral losses, creating a unique fragmentation pattern, which can be used as a fingerprint for members of these natural product classes. The culture supernatants of 69 strains of the were analyzed by MALDI-MS2, and a database comprising MS2 data from each strain was established. This database was scanned for concordant fragmentation patterns of different compounds using a customized software, focusing on relative mass differences of the fragment ions to their precursor ion. A novel group of related natural products comprising 25 different arginine-rich peptides from 16 different strains was identified due to its characteristic neutral loss fragmentation pattern, and the structures of eight compounds were elucidated. Two biosynthesis gene clusters encoding nonribosomal peptide synthetases were identified, emphasizing the possibility to identify a group of structurally and biosynthetically related natural products based on their neutral loss fragmentation pattern.
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