期刊
ANALYTICAL CHEMISTRY
卷 84, 期 19, 页码 8110-8113出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac302210v
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资金
- KHT RD Project
- NRF [2010-0018921, 2011-0018396, 2012-0005860, 2011-0028663]
- Korean Government
- NSF of China [21075094]
- Science Fund for Creative Research Groups [20621502, 20921062]
- Ministry of Health Welfare [A111182]
- National Research Foundation of Korea [2011-0028663] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg2+ ([Mg2+](i)) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca2+ ([Ca2+](m)), FMg2 allows dual-color imaging of Mg2+/Ca2+ activities in live cells and [Mg2+](i)/[Ca2+](m) distributions in live tissues at a depth of 100-200 mu m.
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