期刊
ANALYTICAL CHEMISTRY
卷 84, 期 24, 页码 10645-10649出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac302236a
关键词
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资金
- National Natural Science Foundation of China [20705016, 20975061]
- Independent Innovation Foundation of Shandong University [2012TS002]
- Qilu Hospital of Shandong University
A sandwich-typed near-infrared (NIR) electro-chemiluminescence (ECL) immunoassay was developed with dual-stabilizer-capped CdTe nanocrystals (NCs) as ECL labels and a fetoprotein antigen (AFP) as model protein. The dual-stabilizer-capped NIR CdTe NCs were promising ECL labels because of their NIR ECL emission of 800 nm, low anodic ECL potential of +0.85 V, and high biocompatibity, which can facilitate interference-free and highly sensitive ECL bioassays. Upon the immunorecognition of the immobilized AFP to its antibody labeled with dual-stabilizer-capped CdTe NCs, the proposed immunoassay displayed increasing ECL intensity, leading to a wide calibration range of 10.0 pg/mL to 80.0 ng/mL with a detection limit of 5.0 pg/mL [signal-to-noise ratio (SIN) = 3] without coupling any signal amplification procedures. The NIR ECL immunoassay for real samples displayed very similar results with those of Ru(bpy)(3)(2+) reagent kit based commercial ECL immunoassay, which not only proved for the efficiency of NIR ECL from dual-stabilizer-capped CdTe NCs but also paved the road for development of novel ECL emitters and corresponding reagent kits.
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