期刊
ANALYTICAL CHEMISTRY
卷 84, 期 2, 页码 893-900出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac201179t
关键词
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资金
- National ESCA
- Surface Analysis Center for Biomedical Problems (NESAC/BIO) through National Institutes of Health at the University of Washington [EB-002027]
- University of Washington Engineered Biomaterials 21st Century (UWEB21) center, an Engineering Research Center
Tissue engineering approaches fabricate and subsequently, implant cell-seeded and unseeded scaffold biomaterials. Once in the body, these biomaterials are repopulated with somatic cells of various phenotypes whose identification upon explantation can be expensive and time-consuming. We show that imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) can be used to distinguish mammalian cell types in heterogeneous cultures. Primary rat esophageal epithelial cells (REEC) were cultured with NIH 3T3 mouse fibroblasts on tissue culture polystyrene and freeze-dried before TOF-SIMS imaging. Results show that a short etching sequence with C-60(+) can be used to clean the sample surface and improve the TOF-SIMS image quality. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used to identify peaks whose contributions to the total variance in the multivariate model Were due to either the two cell types or the substrate. Using PLS-DA, unknown regions of cellularity that were otherwise unidentifiable by SIMS could be classified. From the loadings in the PLS-DA model, peaks were selected that were indicative of the two cell types and TOF-SIMS images were created and overlaid that showed the ability of this method to distinguish features visually..
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