期刊
CELL CYCLE
卷 6, 期 13, 页码 1613-1620出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/cc.6.13.4357
关键词
cancer; cell cycle; chloride channels; chromatin condensation; intracellular chloride; glioma; proliferation; volume regulation
类别
资金
- NCI NIH HHS [P50-CA97247, P50 CA097247, P50 CA097247-05] Funding Source: Medline
- NINDS NIH HHS [R01 NS031234-14A1, R01 NS036692-08, R01-NS313234, R01-NS36692, R01 NS036692, R01 NS031234] Funding Source: Medline
Cell growth and osmotic volume regulation are undoubtedly linked to the progres sion of the cell cycle as with each division, a newly generated cell must compensate for loss of half of its volume to its sister cell. The extent to which size influences cell cycle decisions, however, is controversial in mammalian cells. Further, a mechanism by which cells can monitor and therefore regulate their size has not been fully elucidated. Despite an ongoing debate, there have been few studies which directly address the question in single cell real - time experiments. In this study we used fluorescent time - lapse imaging to quantitatively assess volume in individual spontaneously dividing cells throughout the cell cycle. Together with biophysical studies, these establish that the efflux of salt and water brings about a condensation of cytoplasmic volume as glioma cells progress through mitosis. As cells undergo this pre -mitotic condensation ( PMC) they approach a preferred cell volume preceding each division. This is functionally linked to chromatin condensa tion, suggesting that PMC plays an integral role in mitosis.
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