期刊
ANALYTICAL CHEMISTRY
卷 84, 期 17, 页码 7393-7399出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac301101b
关键词
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资金
- Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences of the U.S. Department of Energy [DE-FG02-06ER15758]
- Robert A. Welch Foundation [F-0032]
- project HLRB pr26wo
- U.S. Department of Energy (DOE) [DE-FG02-06ER15758] Funding Source: U.S. Department of Energy (DOE)
We have previously demonstrated up to 5 X 10(5)-fold enrichment of anionic analytes in a microchannel using a technique called bipolar electrode focusing (BEF). Here, we demonstrate that BEF can also be used to enrich a cationic fluorescent tracer. The important point is that chemical modification of the microchannel walls enables reversal of the electroosmotic flow (EOF), enabling cations, instead of anions, to be enriched via an electric field gradient focusing mechanism. Reversal of the EOF has significant consequences on the formation and shape of the region of the buffer solution depleted of charge carriers (depletion zone). Electric field measurements and numerical simulations are used to elucidate the factors influencing the depletion zone. This information is used to understand and control the location and shape of the depletion zone, which in turn influences the stability and concentration of the enriched band.
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