期刊
ANALYTICAL CHEMISTRY
卷 84, 期 6, 页码 2647-2653出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac202559w
关键词
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资金
- KME/KEITI [E211-41003-0007-0, 10035501]
- The Swedish Research Council [621-2009-5361]
- STINT [IG2010 2068]
- KME
- KEIT [S1072576]
- Seoul RBD program [SS100003]
- International Collaborative Research and Development program [GT-2009-ME-DI-0076]
- NRF [KRF-2009-353-D00004]
- Korea Environmental Industry & Technology Institute (KEITI) [ARQ2011410037002] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Korea Evaluation Institute of Industrial Technology (KEIT) [S1072576] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Ministry of Knowledge Economy (MKE), Republic of Korea [GT-2009-ME-DI-0076] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [353-2009-1-D00004] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This paper reports for the first time the application of sol-gel microarrays for immobilizing nonsoluble small chemicals (Bisphenol-A; BPA). Also, known problems of sol-gel adhesion to conventional microtiter well plate substrates are circumvented by anchoring the sol-gel microspots to a porous silion surface so-called, PS-SG chips. We confirmed low molecular weight chemical immobilization inside a sol-gel network using fluorescein. BPA and the BPA specific aptamer were utilized as a model pair to verify the affinity specific interaction in the PS-SG selection system. The aptamer interacted specifically with BPA in the sol-gel spots, as shown in microarrays forming the letters L, U, N, and D. Moreover, the bound aptamer was released by heat, recovered, and verified by gel electrophoresis. The developed PS-SG chip platform will be used for screening aptamers against numerous small molecules such as toxins, metabolites, or pesticide residues.
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