期刊
ANALYTICAL CHEMISTRY
卷 84, 期 18, 页码 7692-7699出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac3015164
关键词
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资金
- National Natural Science Foundation of China [21273117, 21175067]
- Research Fund for the Doctoral Program of Higher Education of China [20103207110004]
- Natural Science Foundation of Jiangsu Province [BK2011779]
- Foundation of the Jiangsu Education Committee [09KJA150001, 10KJB150009]
- Program for Outstanding Innovation Research Team of Universities in Jiangsu Province
- Priority Academic Program Development of Jiangsu Higher Education Institutions
The aptamer (S2.2)-guided Ag-Au nanostructures (aptamer Ag-Au) have been synthesized by photoreduction and validated by ultraviolet-visible light (UV-vis) spectra and transmission electron microscopy (TEM) images. Differential interference contrast (DIC), fluorescence, and TEM images, and surface-enhanced Raman scattering (SERS) spectra indicated that the aptamer-Ag-Au nanostructures can target the surface of human breast cancer cells (MCF-7) with high affinity and specificity. This targeting is completed via the specific interaction between S2.2 aptamer (a 25-base oligonucleotide) and MUC1 mucin (a large transmembrane glycoprotein, whose expression increased at least 10-fold at MCF-7 cells in primary and metastatic breast cancers). However, the nanostructures cannot target HepG2 (human liver cancer cells) or MCF-10A cells (human normal breast epithelial cells), because these cells are MUC1-negative expressed. Moreover, the synthesized nanostructures exhibited a high SERS activity. Based on these results, a new assay for specifically detecting MCF-7 cells has been proposed. This assay can also discriminate MCF-7 cells from MCF-10A cells and different cancer cell lines, such as HepG2 cells. In addition, the aptamer-Ag-Au nanostructures have a high capability of adsorpting near-infrared (NIR) irradiation and are able to perform photothermal therapy of MCF-7 cells at a very low irradiation power density (0.25 W/cm(2)) without destroying the healthy cells and the surrounding normal tissue. Therefore, the proposed assay is significant for the diagnosis of tumors in their nascent stage. The synthesized nanostructures could offer a protocol to specifically recognize and sensitively detect the cancer cells, and would have great potential for application in the photothermal therapy of the cancers.
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