期刊
ANALYTICAL CHEMISTRY
卷 84, 期 3, 页码 1623-1629出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac2029002
关键词
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资金
- National Basic Research Program 973 [2011CB933600, 2010CB732600]
- National Natural Science Foundation of China [21075129]
- Chinese Academy of Science [KGCX2-YW-130]
- Award for the Innovation Team Project of Guangdong Province
- Natural Science Foundation of Shenzhen City [JC201005270327A]
Highly sensitive detection of proteins is essential to biomedical research as well as clinical diagnosis. However, so far most detection methods rely on antibody-based assays and are usually laborious and time-consuming with poor sensitivity. Here, we develop a simple and sensitive method for the detection of a biomarker protein, platelet-derived growth factor BB (PDGF-BB), based on aptamer-based target-triggering two-stage amplification. With the involvement of an aptamer-based probe and an exponential amplification reaction (EXPAR) template, our method combines strand displacement amplification (SDA) and EXPAR, transforming the probe conformational change induced by target binding into two-stage amplification and distinct fluorescence signal. This detection method exhibits excellent specificity and high sensitivity with a detection limit of 9.04 X 10(-13) M and a detection range of more than 5 orders of magnitude, which is comparable with or even superior to most currently used approaches for PDGF-BB detection. Moreover, this detection method has significant advantages of isothermal conditions required, simple and rapid without multiple separation and washing steps, low-cost without the need of any labeled DNA probes. Furthermore, this method might be extended to sensitive detection of a variety of biomolecules whose aptamers undergo similar conformational changes.
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