期刊
ANALYTICAL CHEMISTRY
卷 84, 期 4, 页码 1804-1808出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac203303t
关键词
-
资金
- PRIME-XS [262067]
- European Union
- Netherlands Proteomics Centre, Netherlands Genomics Initiative
- Centre for Biomedical Genetics
- Netherlands Organization for Scientific Research (NWO) [700.10.429]
We present a straightforward method to enrich phosphopeptides with multiple basic residues, an under-represented class in common enrichment strategies. Our method is based on a two-dimensional strong cation exchange (SCX) strategy, operating at two different acidic pHs, enabling both separation and enrichment of different classes of phosphopeptides. The principle of enrichment is based on the change of net charge of phosphorylated peptides under strong acidic conditions in the second SCX, whereas the net charge of regular peptides remains unchanged, thus enabling separation based on net charge. Application of our tandem SCX approach to a modest amount of human cells allowed the identification of over 10 000 unique basic phosphopeptides of which many represent putative targets of basophilic kinases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据