期刊
INTERNATIONAL JOURNAL FOR PARASITOLOGY
卷 37, 期 8-9, 页码 1013-1022出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2007.02.010
关键词
microsatellite; heterozygosity; linkage disequilibrium; relapse; inbreeding; recombination; association mapping
类别
资金
- NCRR NIH HHS [C06 RR013556] Funding Source: Medline
- NIAID NIH HHS [R01 AI48071] Funding Source: Medline
- Wellcome Trust [066439/z/01/2] Funding Source: Medline
Populations of Plasmodium falciparum show striking differences in linkage disequilibrium, population differentiation and diversity, but only fragmentary data exists on the genetic structure of Plasmodium vivax. We genotyped nine tandem repeat loci bearing 2-8 bp motifs from 345 P. vivax infections collected from three Asian countries and from five locations in Colombia. We observed 9-37 alleles per locus and high diversity (H-e = 0.72-0.79, mean = 0.75) in all countries. Numbers of multiple clone infections varied considerably: these were rare in Colombia and India, but >60% of isolates carried multiple alleles in at least one locus in Thailand and Laos. However, only one or two of the nine loci show >1 allele in many samples, suggesting that mutation within infections may result in overestimation of true multiple carriage rates. Identical nine-locus genotypes were frequently found in Colombian populations, contributing to strong linkage disequilibrium. These identical genotypes were strongly clustered in time, consistent with epidemic transmission of clones and subsequent breakdown of allelic associations, suggesting high rates of inbreeding and low effective recombination rates in this country. In contrast, identical genotypes were rare and loci were randomly associated in all three Asian populations, consistent with higher rates of outcrossing and recombination. We observed low but significant differentiation between different Asian countries (standardized, F-ST =0.13-0.45). In comparison, we see greater differentiation between collection locations within Colombia (standardized F-ST = 0.4-0.7), and strong differentiation between continents (standardized F-ST = 0.48-0.79). The observed heterogeneity in multiple clone carriage rates, linkage disequilibrium and population differentiation are similar in some, but not all, respects to those observed in P. falciparum, and have important implications for the design of association mapping studies, and interpretation of P. vivax epidemiology. (C) 2007 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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