期刊
LIVESTOCK SCIENCE
卷 110, 期 3, 页码 280-287出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.livsci.2006.11.009
关键词
energy expenditure; C-13 bicarbonate technique; indirect calorimetry; bulls
We explored the applicability of the 13 C bicarbonate dilution technique for determination of energy expenditure (EE) in young bulls in comparison to whole body indirect calorimetry (IC). Twelve bulls of a F2 German Holstein x Charolais cross (4.5 months, 332 +/- 16 kg BM) received a diet providing 1000 kj ME d(-1) kg BM-0.75 and 4.3 g crude protein d(-1) kg BM-(0.75). Bulls were housed in respiration chambers and received an intravenous bolus of NaH13 CO3 (A:3 mu mol kg BM-1 (n = 2), B: 7 mu mol kg BM-1 (n=4), C: 17.5 mu mol kg BM-1 (n=6),99 at.% C-13) into the jugular vein to measure EE. Simultaneously, EE was determined by IC. After the C-13 administration blood samples and breath gas were collected from the animals in the respiration chamber during a 24-h period (7.00-7.00 h). The recovery of C-13 in breath CO2 (% of C-13 dose) was irrespective of NaH (CO3)-C-13 dose (A: 69.7 +/- 2.7%, B: 70.5 +/- 4.5%, C: 75.0 +/- 4.9%; P > 0.05). Only small amounts of C-13 were excreted in urine (3.4 +/- 2.6%) and feces (2.0 +/- 1.3%). The EE determined by the C-13 bicarbonate method using breath and blood C-13 recovery rates as correction factors was not different from that measured by IC (816 +/- 81 [blood] or 827 +/- 101 [breath] vs. 820 +/- 90 kJ d(-1) kg BM-0.75). Bland-Altman analysis showed a 95% confidence interval for EE of +/- 99 and +/- 109 kJ d(-1) kg BM-0.75 based on blood and breath C-13 recovery, respectively. In conclusion, the 13 C bicarbonate dilution method is appropriate to obtain reliable estimates of EE in young bulls using blood CO2 or breath CO2 under standardized experimental conditions, i.e. in the fasting state. (c) 2006 Elsevier B.V. All rights reserved.
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