期刊
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
卷 133, 期 7, 页码 493-500出版社
SPRINGER
DOI: 10.1007/s00432-007-0193-1
关键词
LNCaP cells; dexamethasone; RU486; ursolic acid; prostate cancer
类别
Purpose Glucocorticoids are widely used as adjuvant therapy in hormonal refractory prostate cancer; their therapeutic role, however, remains unclear. Ursolic acid, a natural triterpene, structurally similar to dexamethasone, exhibits antitumor effects in various cell types. Our main objective was to investigate the effects of ursolic acid on cell viability, apoptosis and bcl-2 protein, in human hormone refractory and androgen-sensitive prostate cancer cells. Methods The ursolic acid-induced changes in cell viability, apoptosis and bcl-2 protein were examined in human hormone refractory prostate cancer PC-3 cells and androgen-sensitive LNCaP cells, by MTT assay, flow cytometry and western blot analysis, respectively. Results Ursolic acid inhibited significantly the cell viability and induced apoptosis in PC-3 cells at 55 mu M and in LNCaP cells at 45 mu M associated with a downregulation of bcl-2 protein. Conclusions The antiproliferative and apoptotic effects of ursolic acid in PC-3 and LNCaP cells implicate its potential therapeutic use for the treatment of hormone refractory and androgen-sensitive prostate cancer. The downregulation of bcl-2 may be one of the molecular mechanisms via which it induces apoptosis in PC-3 and LNCaP cells.
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