期刊
ANALYTICAL CHEMISTRY
卷 83, 期 6, 页码 2020-2028出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac2001737
关键词
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资金
- National Institutes of Health [EB008392, HL092836, EB009196, DE019024]
- National Science Foundation [DMR0847287]
- Institute for Soldier Nanotechnology
- Office of Naval Research
- US Army Corps of Engineers
- Korean Government [KRF-2008-357-D00099]
- Ministry of Education, Science and Technology [2010-0005219]
- National Research Foundation of Korea [2010-0005219, 2008-357-D00099] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We describe a microfluidic device for generating nonlinear (exponential and sigmoidal) concentration gradients, coupled with a microwell array for cell storage and analysis. The device has two inputs for coflowing multiple aqueous solutions, a main coflow channel and an asymmetrical grid of fluidic channels that allows the two solutions to combine at intersection points without fully mixing. Due to this asymmetry and diffusion of the two species in the coflow channel, varying amounts of the two solutions enter each fluidic path. This induces exponential and sigmoidal concentration gradients at low and high flow rates, respectively, making the microfluidic device versatile. A key feature of this design is that it is space saving, as it does not require multiplexing or a separate array of mixing channels. Furthermore, the gradient structure can be utilized in concert with cell experiments, to expose cells captured in microwells to various concentrations of soluble factors. We demonstrate the utility of this design to assess the viability of fibroblast cells in response to a range of hydrogen peroxide (H2O2) concentrations
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