期刊
ANALYTICAL CHEMISTRY
卷 83, 期 19, 页码 7283-7289出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac201602s
关键词
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资金
- Japanese society for the Promotion of Science, Japan
- Japan Science and Technology Agency
- Human Metabolomics Technology
- Grants-in-Aid for Scientific Research [20670004] Funding Source: KAKEN
We report the detection of a group of endogenous low molecular weight metabolites (LMWM) in mouse brain (80-500 Da) using TiO2 nanoparticles (NPs) in nanoparticle-assisted laser desorption/ionization-imaging mass spectrometry (Nano-PALDI-IMS) without any washing and separation step prior to MS analysis. The identification of metabolites using TiO2 NPs was compared with a conventional organic matrix 2,5-dihydroxybenzoic acid (DHB) where signals of 179 molecules were specific to TiO2 NPs, 4 were specific to DHB, and 21 were common to both TiO2 NPs and DHB. The use of TiO2 NPs enabled the detection of a higher number of LMWM as compared to DHB and gold NPs as a matrix. This approach is a simple, inexpensive, washing, and separation free for imaging and identification of LMWM in mouse brain. We believe that the biochemical information from distinct regions of the brain using a Nano-PALDI-IMS will be helpful in elucidating the imbalances linked with diseases in biomedical samples.
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