期刊
ANALYTICAL CHEMISTRY
卷 83, 期 19, 页码 7276-7282出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac200617k
关键词
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资金
- National Basic Research Program of China [2010CB732403]
- National Natural Science Foundation of China [20975023, 20735002]
- program for new century excellent talents in university of China [09-0014]
- National Science Foundation of Fujian Province [2010J06003]
Peptide-protein interactions have critical roles in biology. Monitoring peptide protein interactions plays an important role in investigating molecular recognition, screening drugs, and designing biosensors. In this paper, we develop a novel fluorescent approach to monitor peptide protein interactions based on the assembly of pyrene-labeled peptide and graphene oxide (GO). The pyrene-labeled peptide is strongly adsorbed on the surface of GO via pi-pi interactions and hydrophobic interactions. As a result, the proximity of the GO to the pyrene moiety effectively quenches the fluorescence of pyrene. In the presence of target protein, the competitive binding of the target protein with GO for peptide results in the restoration of fluorescence signal. This signaling mechanism makes it possible to monitor the peptide protein interactions in a homogeneous real-time format.
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