期刊
ANALYTICAL CHEMISTRY
卷 83, 期 5, 页码 1862-1865出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac103086v
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资金
- Deutsche Forschungsgemeinschaft (DFG) [Se 777/9-1, MA 3257/2-1]
- BMBF [01 GS 08143]
- Forum (Forschungsforderung Ruhr-Universitat Bochum Medizinische Fakultat) [F656-2009]
Novel molecularly imprinted polymers (MIPs) designed to bind the side chain of phosphotyrosine can be used as artificial receptors for affinity-based enrichment of proteolytic peptides. In comparison with general enrichment methods for phosphorylated peptides such as TiO(2)-based methods, the pTyr-imprinted polymers offered high selectivity for pTyr-containing peptides down to the low fmol level. This suggests MIPs as a new tool for affinity-based proteomics.
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