期刊
ANALYTICAL CHEMISTRY
卷 82, 期 9, 页码 3588-3596出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac100621r
关键词
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资金
- National Natural Science Foundation of China [20673057, 20773067, 20833006, 20905036]
- Program for New Century Excellent Talents in University [NET-06-0508]
- Foundation of the Jiangsu Education Committee [09KJA150001, 09KJB150006]
This work develops a novel electrochemical approach for detection of the extracellular oxygen released from human erythrocytes. The sensing is based on the bioelectrocatalytic system of graphene integrated with laccase (Lac) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) toward the reduction of oxygen. ABTS and laccase are assembled on the surface of graphene, which is synthesized by a chemistry route, utilizing the pi-pi and electrostatic interactions of these components. Transmission electron microscopy (TEM), atomic force microscopy (AFM), and FT-IR spectroscopy demonstrate that graphene has been successfully synthesized, and ABTS and laccase have been effectively assembled on a graphene surface with the formation of Lac-ABTS-graphene hybrid. The voltammetric results indicate that ABTS can be used as a redox mediator when it is in immobilized form. The hybrid deposited on the glassy carbon (GC) electrode is demonstrated to be a good bioelectrocatalyst for the reduction of oxygen with inherent enzyme activity, accepted stability, high half-wave potential (ca.670 mV vs NHE), and unimpeded electrical communication to the copper redox sites of laccase. Therefore, this study has not only established a novel approach of detection of extracellular oxygen but also provided a general route for fabricating a graphene-based biosensing platform via assembling enzymes/proteins on a graphene surface.
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