期刊
ANALYTICAL CHEMISTRY
卷 82, 期 20, 页码 8510-8518出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac101388b
关键词
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资金
- Korean Ministry of Education, Science Technology [FPR08A1-010]
- Korea Research Foundation [KRF-2009-013-C00036]
- Converging Research Center through the Ministry of Education, Science and Technology [2010K001300]
- NIH National Center for Research Resources [RR18522]
Accurate assignment of monoisotopic precursor masses to tandem mass spectrometric (MS/MS) data is a fundamental and critically important step for successful peptide identifications in mass spectrometry based proteomics. Here we describe an integrated approach that combines three previously reported methods of treating MS/MS data for precursor mass refinement. This combined method, integrated post-experiment monoisotopic mass refinement (iPE-MMR), integrates steps (1) generation of refined MS/MS data by DeconMSn; (2) additional refinement of the resultant MS/MS data by a modified version of PE-MMR; and (3) elimination of systematic errors of precursor masses using DtaRefinery. iPE-MMR is the first method that utilizes all MS information from multiple MS scans of a precursor ion including multiple charge states, in an MS scan, to determine precursor mass. With the combination of these methods, iPE-MMR increases sensitivity in peptide identification and provides increased accuracy when applied to complex high-throughput proteomics data.
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