期刊
PEPTIDES
卷 28, 期 7, 页码 1424-1432出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2007.05.007
关键词
Alzheimer's disease; amyloid beta peptide; copper; BBB
The amyloid hypothesis states that amyloid beta protein (A beta) plays a major causal role in the onset of Alzheimer's disease. Toxicity of A beta can be modified by metal ions. Two mechanisms by which such A beta and metal ions could interact are by enhanced oxidative stress or by altered fibrillation. Specifically, A beta fibrillation is increased by aluminum (Al) and copper (Cu) and Al also increases A beta uptake into brain. Here, we determined whether chelation with Cu would alter uptake of the human or rat 1-42 form of A beta (A beta 42) by brain or alter A beta-induced oxidative stress in an immortalized line of rat brain endothelial cells (RBE4). We found that Cu enhanced cytotoxicity of rat, but not of human A beta, had no effect on glutathione (GSH) or cysteine (CYS) levels. Cu significantly decreased homocysteine (HCYS) levels when complexed with A beta. Cu chelation did not alter A beta uptake into brain or other tissues (except for kidney) or alter clearance from blood or brain in vivo, but did increase efflux in an in vitro model of the blood-brain barrier (BBB). Chelation to Cu also impaired the capillary to brain transport of A beta, an effect opposite to that previously found for chelation of A beta to Al. These results show that metal ions have varied effects on A beta uptake by brain and that Cu could be protective against the neurotoxic effects of circulating A beta. (C) 2007 Elsevier Inc. All rights reserved.
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