The sensor exploits the phenomenon of upconversion luminescence and is based on (a) the use of upconverting nanoparticles (UCNPs) of the NaYF4:Yb,Er type that can be excited with 980 nm laser light to give a green and red luminescence and (b) the pH probe phenol red immobilized in a polystyrene matrix. Exposure of the sensor film to ammonia causes a strong increase in the 560 nm absorption of the pH probe which, in turn, causes the green emission of the UCNPs to be screened off. The red emission of the UCNPs, in contrast, remains unaffected by ammonia and can serve as a reference signal. Due to the use of 980 nm as the excitation light source, the optical signal obtained is completely free of background visible luminescence of the sample and of scattered light. This is highly advantageous in the case of sensing ammonia in complex matrixes.
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