期刊
ANALYTICAL CHEMISTRY
卷 82, 期 3, 页码 1039-1046出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac902346a
关键词
-
资金
- The Netherlands Bioinformatics Centre (NBIC) [SP 3.3.1]
- Spinozapremie [NWO/SPI 56-464-14192]
- Center for Medical Systems Biology (CMSB)
- NWO-MaGW [480-04-004]
- NWO-MaGW Vervangingsstudie (NWO) [40005-717]
Combination of data sets from different objects (for example, from two groups of healthy volunteers from the same population) that were measured on a common set of variables (for example, metabolites or peptides) is desirable for statistical analysis in omics studies because it increases power. However, this type of combination is not directly possible if nonbiological systematic differences exist among the individual data sets, or blocks. Such differences can, for example, be due to small analytical changes that are likely to accumulate over large time intervals between blocks of measurements. In this article we present a data transformation method, that we,will refer to as quantile equating, which per variable corrects for linear and nonlinear differences in distribution among blocks of semiquantitative data obtained with the same analytical method. We demonstrate the successful application of the quantile equating method to data obtained on two typical metabolomics platforms, i.e., liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy. We suggest uni- and multivariate methods to evaluate similarities and differences among data blocks before and after quantile equating. In conclusion, we have developed a method to correct for nonbiological systematic differences among semiquantitative data blocks and have demonstrated its successful application to metabolomics data sets.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据