4.5 Article

Characteristics of skeletal muscle mitochondrial biogenesis induced by moderate-intensity exercise and weight loss in obesity

期刊

JOURNAL OF APPLIED PHYSIOLOGY
卷 103, 期 1, 页码 21-27

出版社

AMER PHYSIOLOGICAL SOC
DOI: 10.1152/japplphysiol.01228.2006

关键词

mitochondria; cardiolipin

资金

  1. NCATS NIH HHS [UL1 TR000005] Funding Source: Medline
  2. NCRR NIH HHS [5-M01-RR-00056] Funding Source: Medline
  3. NIA NIH HHS [R01 AG021961] Funding Source: Medline
  4. NIDDK NIH HHS [P30DK462, R01-DK-49200-06] Funding Source: Medline

向作者/读者索取更多资源

There are fewer mitochondria and a reduced oxidative capacity in skeletal muscle in obesity. Moderate-intensity physical activity combined with weight loss increase oxidative enzyme activity in obese sedentary adults; however, this adaptation occurs without a significant increase in mitochondrial DNA (mtDNA), which is unlike the classic pattern of mitochondrial biogenesis induced by vigorous activity. The objective of this study was to examine the hypothesis that the mitochondrial, adaptation to moderate-intensity exercise and weight loss in obesity induces increased mitochondrial cristae despite a lack of mtDNA proliferation. Content of cardiolipin and mtDNA and enzymatic activities of the electron transport chain (ETC) and tricarboxylic acid (cycle were measured in biopsy samples of vastus lateralis muscle obtained from sedentary obese men and women before and following a 4-mo walking intervention combined with weight loss. Cardiolipin increased by 60% from 47 +/- 4 to 74 8 mu g/mU CK (P < 0.01), but,skeletal muscle mtDNA content did not change significantly (1,901 363 to 2,169 317 Rc, where Re is relative copy number of mtDNA per diploid nuclear genome). Enzyme activity of the ETC increased (P < 0.01); that for rotenone-sensitive NADH-oxidase (96 +/- 1%) increased more than for ubiquinol-oxidase (48 +/- 6%). Activities for citrate synthase and succinate dehydrogenase increased by 29 +/- 9% and 40 6%, respectively. In conclusion, moderate-intensity physical activity combined with weight loss induces skeletal muscle mitochondrial biogenesis in previously sedentary obese men and women, but this response occurs without mtDNA proliferation and may be characterized by an increase in mitochondrial cristae.

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