期刊
ANALYTICAL CHEMISTRY
卷 82, 期 17, 页码 7178-7184出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac101892t
关键词
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资金
- National 863 Program [2007AA03Z357]
- National Natural Science Foundation of China [20975026]
- Shanghai Key Basic Research Program [08JC1402600]
- Research Fund for the Doctoral Program of Higher Education [20090071110056]
Short-length DNA and RNA, such as mature small RNA, which contains only 17-25 nucleotides, are always a problem in hybridization-based detection assays. In this paper, we report a proof-of-concept for a new short-length DNA detection technology which encompasses a design strategy whereby capture and reporter probes that do not hybridize to each other at 20 degrees C can be made to anneal to each other in the presence of a template via the formation of a stable three-component complex. The thermodynamics of this magnetic bead-based DNA biosensor was then investigated in detail by monitoring chemiluminescence (CL) changes in the absence and presence of targets over a temperature profile. The data show that this new biosensor offers the possibility of highly selective and sensitive detection of the short-length target DNA. In view of these advantages, this template-dependent surface-hybridization assay, as a new CL strategy, might create a universal technology for developing simple biosensors in sensitive and selective detection of short-length DNA and RNA.
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