期刊
ANALYTICAL CHEMISTRY
卷 82, 期 4, 页码 1470-1477出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac902602e
关键词
-
资金
- Swedish Research Council [7461, 21027, 342-2004-4434]
- Swedish Cancer Foundation
- Knutand Alice Wallenberg Foundation [KAW2007.0118]
- IngaBritt and Arne Lundberg Foundation
- Sahlgren's University Hospital
- EU-FP7 IBDase
- Wilhelm and Martina Lundgren's Foundation
- Swedish Foundation for Strategic Research-The Mucosal Immunobiology and Vaccine Center (MI-VAC)
Negative ion mode nanoliquid chromatography/mass spectrometry (nano-LC/MS) on porous graphitic carbon columns at pH 11 was studied and compared to capillary LC/MS at pH 8 for the analysis of neutral and acidic glycan alditols. Oligosaccharides were chromatographed with an acetonitrile gradient containing 0.04% ammonium hydroxide and analyzed with a linear ion trap mass spectrometer (LTQ) equipped with a modified nanospray interface. Analysis of acidic N- and O-glycan standards revealed that good quality MS/MS spectra could be obtained when loading 1-3 fmol, a 10-fold increase in sensitivity compared to capillary-LC/MS at pH 8. Analysis of a complex mixture of O-glycans from porcine colonic mucins with nano-LC/MS and MS/MS at high pH revealed 170 oligosaccharides in one analysis, predominantly corresponding to sulfated glycans with up to 11 residues. Analysis of the same sample with capillary-LC/MS showed a lower sensitivity for multiply sulfated glycans. Nano-LC/MS of O-linked oligosaccharides on MUC2 from a human colon biopsy also illustrated that the ionization of oligosaccharides with multiple sialic acid groups was increased compared to those with only one sialic acid residue. Nano-LC/MS at high pH is, thus, a highly sensitive approach for the analysis of acidic oligosaccharides.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据