期刊
ANALYTICAL CHEMISTRY
卷 82, 期 23, 页码 9622-9625出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac1023099
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资金
- National Basic Research Program of China [2007CB914100]
- National Natural Science Foundation [20935004]
- Chinese Academy of Sciences [KJCX2YW H09]
In this study, a facile system for membrane proteome profiling was established, in which membrane proteins were solubilized by formic acid, online digested by a pepsin-based immobilized enzyme reactor (pepsin IMER), and analyzed by strong cation exchange and microflow reversed phase liquid chromatography with electrospray ionization tandem mass spectrometry (SCX-mu RPLC-ESI-MS/MS) Under optimized conditions, such a system showed excellent compatibility between all crucial steps and was successfully applied for analyzing integral membrane proteins extracted from rat liver microsomes Out of the 235 unique proteins positively identified, 39% (91/235) were annotated as membrane proteins with one or more transmembrane domains (TMDs) It is anticipated that the efficient sample treatment and the relevant online analytical system might provide a promising tool for automated and comprehensive profiling of membrane proteomes
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