期刊
MOLECULAR REPRODUCTION AND DEVELOPMENT
卷 74, 期 7, 页码 843-850出版社
WILEY
DOI: 10.1002/mrd.20685
关键词
non-genomic receptor; ciliated cells; gamete transport; reproduction; oviduct
资金
- NIEHS NIH HHS [ES12961] Funding Source: Medline
Non-genomic, rapid actions of steroids have long been known, suggesting the possible presence of non-classical steroid receptors. A membrane receptor for progestins (mPR) was recently described in the spotted seatrout, and transcripts of three related receptors (alpha, beta, and gamma) were subsequently identified in other species including human and mouse. To begin exploring the roles of mPR gamma in mammals, we have generated an antibody against this receptor. The specificity of the antibody was demonstrated by both overexpression and RNA interference experiments. Using the antibody, we show that mPR gamma is expressed in female mouse reproductive tissues such as ovary and fallopian tube, and also in the lung and liver of both sexes. Immunohistochemical studies revealed that mPR gamma is associated with the apical membrane of ciliated cells facing the lumen of the fallopian tube. The presence of mPR gamma in ciliated cells of the fallopian tube was also demonstrated in human samples. Rapid effects of progesterone on ciliary beat frequency in the fallopian tube have recently been reported. Together, this suggests a common role for mPR gamma in the regulation of ciliary activity in the fallopian tube and thus gamete transport in mammals. The presence of mPR gamma in lung and liver of mice suggests that the receptor mediates the actions of progesterone outside the reproductive tract as well.
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