期刊
ANALYTICAL CHEMISTRY
卷 82, 期 6, 页码 2192-2203出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac9024889
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资金
- National Institute of General Medical Sciences [R01GM086196]
The number of reports per year on single-molecule imaging experiments has grown roughly exponentially since the first successful efforts to optically detect a single molecule were completed over two decades ago. Single-molecule spectroscopy has developed into a field that includes a wealth of experiments at room temperature and inside living cells. The fast growth of single-molecule biophysics has resulted from its benefits in probing heterogeneous populations, one molecule at a time, as well as from advances in microscopes and detectors. This Perspective summarizes die field of live-cell imaging of single biomolecules.
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