期刊
ELECTROANALYSIS
卷 19, 期 13, 页码 1415-1420出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200703872
关键词
biosensor; pesticides; self-assembled monolayer; enzyme; acetylcholinesterase
Studies on the immobilization of acetylcholinesterase onto a SAM gold electrode and the use of the fabricated biosensor for the determination of carbaryl and parathion are presented. The influence of pH, ionic strength, enzyme loading and concentration of glutaraldehyde on the response of the biosensor was investigated. The amperometric biosensor developed in this study provided linearity to parathion and carbaryl in the 2.0 a 30.0 x 10(-6) mol L-1 concentration range. The detection limits under the optimum working conditions were found to be 9.3 mu g L-1 for parathion and 9.0 mu g L-1 for carbaryl. The enzyme electrode was found to be stable for 7 days.
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