期刊
ANALYTICAL CHEMISTRY
卷 82, 期 11, 页码 4333-4336出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac100865p
关键词
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资金
- Ministry of Education, Culture, Sports, Sciences, and Technology of Japan
Sandwich-type noncompetitive (immunometric) assays allow for high-sensitivity high-throughput macromolecule sensing and determination but cannot be used on small molecules (haptens). Here, we isolated single-chain Fv fragments from a phage-display library, which bound to complexes of particular haptens (vitamin D and A derivatives) with immobilized beta-cyclodextrin or beta-maltosyl residues, and formed ternary complexes. These scFvs enabled novel semisandwich-type immunometric assays of haptens with nanomole-range sensitivities.
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